Madhusudhan Reddy Papasani, PhD

Research Scientist

Office Location 316 AG Biotechnology Bldg University of Idaho Moscow, ID 83844-2330 Phone: 208-885-6351 papasani@uidaho.edu

Responsibilities

Managing the growth biology research program in the areas of cellular biology, biotechnology and molecular biology by providing major oversight for the program including providing training and supervision of laboratory staff.  Performs qualitative and quantitative analyses in the areas of cellular biology, biochemistry and molecular biology using zebrafish and other models including primary cells and cell lines derived from various species to understand developmental roles or regulatory pathways controlling energy metabolism, nanomaterials in understanding cell and molecular biology mechanisms and applications.  Current focus :Cloning and structural characterization of genes regulating energy metabolism pathways, quantifying their mRNA expression at different developmental or physiological stages, identifying the tissues that express transcripts of specific gene families, identifying gene regulatory regions, and their cloning, in vitro and in vivo characterization. Applies theoretical principles and develops laboratory protocols; interacts with collaborating faculty and other technical support personnel; and independently tests and modifies established methods and develops new procedures to meet the goals of the laboratories

Teaching

 Instructor for Growth Physiology inquisition AVS 509

Education

PhD in Animal Physiology, Department of Animal & Veterinary Science, University of Idaho, Moscow (2008)                             

  M.S. in Biological sciences emphasis on Biotechnology, Department of Biology, Illinois State University, Normal, IL (2004)

   B.Sc in Horticulture, A.N.G.R. Agricultural University, India (2000)

Appointments

    09/2007-present     Research Scientist, Department of Animal and Veterinary Science,  University of Idaho, Moscow, USA (full time)

  08/2004-08/2007:    Research support scientist, Department of Animal and Veterinary       Science, University of Idaho, Moscow, USA (full time)

   08/2005-05/2008      Graduate Research Assistant, Department of Animal and Veterinary  Science, University of Idaho, Moscow, USA (20 hrs/week)

  01/2002 –05/2004     Graduate  Assistant:  Department of Biology, Illinois State   University, Normal, USA   (20 hrs/week)

Publications

 .    1.  Kokta TA, Strat AL, Papasani MR, Szasz JI, Dodson MV and Hill RA 2008.Regulation of Lipid Accumulation in 3T3-L1 Cells: Effects of Oleic and Linoleic Acids and Insulin.  Animal 2(1)-92-99  

2.  Robison BD, Drew RE, Powell  M, Stone D, Murdoch G, Settles M, Churchill E, Rodnic K , Hill RA, Papasani MR, Lewis S, and Hardy RW 2008. Sexual dimorphism in hepatic gene expression and the response to dietary carbohydrate manipulation in the zebrafish (Danio rerio)  Comparative Biochemistry and Physiology Part D: Genomics and Proteomics 3: 141-154

3.   Papasani MR, Behnke S, Rodgers DB,  Hill RA 2008.  Co-expression of a Second Insulin Gene and its Trans-Activator in Zebrafish: Putative Functions in Novel Tissues ( Submitted to FASEB Journal)

4.  Papasani MR, Behnke S, Gordon GK, Hill RA 2008.  Comparative analyses of insulin gene promoters of zebrafish and humans.  (Submitted to General  Comparative  Endocrinology)

5.  Papasani MR, Robison BD, Hardy RW, Hill RA  2006. Developmental Expression of Two Insulins in Zebrafish.   Physiological Genomics 27: 79-85 (2006)

6.    Papasani MR, Gensure R, Postlethwait JH, Gunes Y, Yan Y.-L,Ponugoti B, Jüppner H, Rubin DA.  2004.   Identification and Characterization of the Zebrafish and Fugu Gene Encoding Tuberoinfundibularpeptide of 39 residues (TIP39).  Endocrinology 145 (11): 5294-5304

7.   Gensure RC, Ponugoti B, Gunes Y, Papasani MR, Bastepe M, RubinDA, Jüppner H. 2004.  Identification and Characterization of Two Parathyroid Hormone-like Molecules in Zebrafish.  Endocrinology 145 (4): 1634 –1639

8.   Papasani MR, Halterman K,  Hill RA 2006 Defining the promoter region of a second insulin gene in zebrafish  Integrative and Comparative Biology, 46: E108-E108 Suppl.

9.   Gensure RC, Ponugoti B, Gunes Y, Papasani MR, Bastepe M, Rubin DA, Juppner H Identification and characterization of two PTH-like molecules in zebrafish 2003. Journal of Bone and Mineral Research, 18: S399-S399 Suppl

10.  Papasani, MR; Gensure, RC; Postlethwait, JH; Gunes, Y; Ponugoti, B; Juppner, H; Rubin, DA 2003.  Identification and characterization of the zebrafish gene encoding tuberoinfundibular peptide of 39 residues. Journal of Bone and Mineral Research, 18: S400-S400 Suppl.

11.  Ponugoti, B; Papasani, MR; Layman, JA; Gensure, R; Juppner, H; Rubin, DA 2002.  Molecular isolation and evolution of teleost parathyroid hormones (PTH), PTH-related peptides (PTHrP) and tuberoinfundibular peptides of 39 residues (TIP39). Integrative and Comparative Biology, 42 (6): 1296-1296

 National and International Scientific Conference Presentations

1.     Papasani MR, Behnke S, Hill RA (2007).  Zebrafish PDX1 regulates promoter  activity of a second insulin gene (insb). Endocrine Society's 90th Annual Meeting, June 15-18, 2007, San Francisco, USA

2.     Papasani MR, Halterman K, Hill RA (2007). Identification and characterization of the  promoter    region of a second insulin gene in zebrafish. Endocrine Society's 89th Annual Meeting , June 2-5, 2007, Toronto, Canada

3.     Papasani MR, Halterman K, Hill RA (2007). Defining the promoter region of a second insulin gene in zebrafish. Society for Integrative and Comparative Biology 2007 Annual Meeting, January  3-7, 2007, Phoenix, AZ, USA

4.     Papasani MR, Robison BD, Hardy RW, Hill RA  (2006). Temporal and spatial expression of two insulin genes in Zebrafish.   Endocrine Society's 88th Annual Meeting , June 24-27, 2006,  Boston, MA , USA

5.     Papasani MR, Robison BD, Hardy RW, Hill RA  (2006). Identification and characterization of a second insulin gene in zebrafish, April 12, 2006 , GPSA Research symposium, University of Idaho,Moscow, ID, USA

6.     Amberg JJ, Papasani MR, Cook MA. Hardy RW, Rust MB, Anderson CL, Hill RA 2006.  An intestinal peptide transporter, pept1-like, identified in altricial larval fishes: beginning to understand the importance of intestinal peptide uptake in larval fishes. World Aqua- culture Society Meeting 2006  February 13 - 16, 2006, Las Vegas, Nevada, USA  

7.     Papasani MR, Kokta TA, Strat AL, Gertler A. and Hill R.A  2005. Regulation of gene expression in Sol8 myogenic cells in response to leptin. The Endocrine Society's 87th Annual Meeting, June 4-7, 2005, San Diego, CA, USA

8.     Kokta TA, Strat AL, Papasani MR, Szasz JI, Dodson MV and Hill RA. 2005.  Regulation of Lipid Accumulation in 3T3-L1 cells: effects of Oleic and Linoleic Acids and Insulin.  The Endocrine Society's 87th Annual Meeting , June 4-7, 2005,  San Diego, CA , USA

9.     Papasani MR, Gensure R, Postlethwait JH, Gunes Y, Yan Y.-L, Ponugoti B, Jüppner H, Rubin DA. 2003. Identification and Characterization of the Zebrafish Gene Encoding Tuberoinfundibular peptide of 39 residues (TIP39). 25th Annual Meeting of the American Society for Bone and Mineral Research,  September19-23, 2003.  Minneapolis, Minnesota, USA

10.     Gensure RC, Ponugoti B, Gunes Y, Papasani MR , Bastepe M, Rubin DA, JüppnerH. 2003. Identification and Characterization of Two PTH-like Molecules in Zebrafish.  25th Annual Meeting of the American Society for Bone and MineraL Research, September 19-23, 2003. Minneapolis, Minnesota, USA  
 

11.     Ponugoti B, Papasani MR, Layman JA, Gensure R, and Jüppner H, and Rubin, DA 2003.  Molecular isolation and evolution of teleost parathyroid hormone (PTH), PTH-related peptide (PTHrP) and tuberoinfundibular peptide of 39 residues (TIP39).  Society for Integrative and Comparative Biology 2003 Annual Meeting, January 6, 2003,Toronto, Canada

Gene bank submissions: (National center for biotechnology information)

1. Papasani MR, Robison BD, Hardy RW, Hill RA 2006  Danio rerio preproinsulin b (insb),mRNA Gene accession no: NM_001039064

2. Papasani,M.R., Gensure,R.C., Yan,Y.L., Gunes,Y., Postlethwait,J.H.,Ponugoti,B., John,M.R., Juppner,H. and Rubin,D.A 2004 . Danio rerio tuberoinfundibular peptide of 39 amino acids (tip39), mRNA  Genebank accession no: NM_205577

3. Papasani,M.R., Gensure,R.C., Yan,Y.L., Gunes,Y., Postlethwait,J.H.,Ponugoti,B., John,M.R.,Juppner,H. and Rubin,D.A 2004  Danio rerioTuberoinfundibular peptide Tip39 splice variant (tip39) mRNA, complete cdsalternatively splice  Gene accession no: AY306196

4. Gensure, R.C., Ponugoti, B., Gunes, Y., Papasani, M.R., Lanske, B., Bastepe, M., Rubin, D.A., and Juppner, H. 2004. Danio rerio parathyroid hormone 1(pth1), mRNA Genebank accession no: NM_212949

5. Gensure,R.C., Ponugoti,B., Gunes,Y., Papasani,M.R., Lanske,B.,Bastepe,M., Rubin,D.A and Juppner,H 2004. Danio rerio parathyroid hormone 1(pth1),mRNA Genebank accession no: NM_212950